Coding

Part:BBa_K2933106:Design

Designed by: Xueqing Fu   Group: iGEM19_TJUSLS_China   (2019-09-13)


GST+Linker+ElBlaII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

ElBlaII is codon optimized for BL21(DE3) E. coli.


Source

NCBI.

References